GeneTex

  • Country / Location Selection

United States (US)

TIGIT antibody [HL2655]

Recombinant antibody Comparison Orthogonal Overexpression
Anti-TIGIT antibody [HL2655] used in Western Blot (WB). GTX639114
Anti-TIGIT antibody [HL2655] used in Western Blot (WB). GTX639114
Anti-TIGIT antibody [HL2655] used in Western Blot (WB). GTX639114
Anti-TIGIT antibody [HL2655] used in Western Blot (WB). GTX639114

Cat. No. GTX639114

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL2655

Isotype

 IgG

Application

 WB

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all TIGIT products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

26 kDa. ( Note )

Product Note

This antibody was raised against human TIGIT Intracellular domain.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the Intracellular domain of human TIGIT. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

T cell immunoreceptor with Ig and ITIM domains , VSIG9 , VSTM3 , WUCAM

Cellular Localization

Cell membrane

Background

This gene encodes a member of the PVR (poliovirus receptor) family of immunoglobin proteins. The product of this gene is expressed on several classes of T cells including follicular B helper T cells (TFH). The protein has been shown to bind PVR with high affinity; this binding is thought to assist interactions between TFH and dendritic cells to regulate T cell dependent B cell responses.[provided by RefSeq, Sep 2009]

Database

Research Area

DATA IMAGES

Anti-TIGIT antibody [HL2655] used in Western Blot (WB). GTX639114

GTX639114 WB Image

Comparison

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membranes were blotted with TIGIT antibody [HL2655] (GTX639114) diluted at 1:1000 and competitor's antibody (Highly Cited Antibody) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-TIGIT antibody [HL2655] used in Western Blot (WB). GTX639114

GTX639114 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with TIGIT antibody [HL2655] (GTX639114) diluted at 1:30000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-TIGIT antibody [HL2655] used in Western Blot (WB). GTX639114

GTX639114 WB Image

Orthogonal

MJ whole cell and membrane extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with TIGIT antibody [HL2655] (GTX639114) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-TIGIT antibody [HL2655] used in Western Blot (WB). GTX639114

GTX639114 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with TIGIT antibody [HL2655] (GTX639114) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

REFERENCE

There are currently no references for TIGIT antibody [HL2655] (GTX639114). Be the first to share your publications with this product.
Submit a Reference

REVIEW

There are currently no reviews for TIGIT antibody [HL2655] (GTX639114). Be the first to share your experience with this product.
Submit a Review
Package List Price ($)
$ 399
$ 169
Custom Conjugations