APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500-1:3000 |
Assay dependent |
Assay dependent |
Not tested in other applications.
Calculated MW
Product Note
This antibody is specific for human VEGFA protein, and it does not cross react with human VEGFB protein.
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant fragment of human VEGFA.
Purification
Affinity purified by Protein A.
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
vascular endothelial growth factor A , MVCD1 , VEGF , VPF
Cellular Localization
Secreted
Background
This gene is a member of the PDGF/VEGF growth factor family. It encodes a heparin-binding protein, which exists as a disulfide-linked homodimer. This growth factor induces proliferation and migration of vascular endothelial cells, and is essential for both physiological and pathological angiogenesis. Disruption of this gene in mice resulted in abnormal embryonic blood vessel formation. This gene is upregulated in many known tumors and its expression is correlated with tumor stage and progression. Elevated levels of this protein are found in patients with POEMS syndrome, also known as Crow-Fukase syndrome. Allelic variants of this gene have been associated with microvascular complications of diabetes 1 (MVCD1) and atherosclerosis. Alternatively spliced transcript variants encoding different isoforms have been described. There is also evidence for alternative translation initiation from upstream non-AUG (CUG) codons resulting in additional isoforms. A recent study showed that a C-terminally extended isoform is produced by use of an alternative in-frame translation termination codon via a stop codon readthrough mechanism, and that this isoform is antiangiogenic. Expression of some isoforms derived from the AUG start codon is regulated by a small upstream open reading frame, which is located within an internal ribosome entry site. [provided by RefSeq, Nov 2015]
Database
Research Area
DATA IMAGES
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GTX637405 ICC/IF Image
VEGF antibody [HL1755] detects VEGF protein at vesicle by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: VEGF stained by VEGF antibody [HL1755] (GTX637405) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.Blue: Fluoroshield with DAPI (GTX30920).
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GTX637405 WB Image
HepG2 whole cell extract and conditioned medium (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with VEGFA antibody [HL1755] (GTX637405) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX637405 WB Image
Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with VEGFA antibody [HL1755] (GTX637405) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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GTX637405 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts were separated by 15% SDS-PAGE, and the membrane was blotted with VEGFA antibody [HL1755] (GTX637405) diluted at 1:500000. The HRP-conjated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX637405 IHC-P Image
VEGFA antibody [HL1755] detects secreted VEGFA protein by immunohistochemical analysis.Sample: Paraffin-embedded cat kidney.VEGFA stained by VEGFA antibody [HL1755] (GTX637405) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX637405 IHC-P Image
VEGF antibody [HL1755] detects VEGF protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded human kidney.VEGF stained by VEGF antibody [HL1755] (GTX637405) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX637405 IHC-P Image
VEGF antibody [HL1755] detects VEGF protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse liver.VEGF stained by VEGF antibody [HL1755] (GTX637405) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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REFERENCE
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REVIEW
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