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Von Hippel Lindau antibody, Internal

Anti-Von Hippel Lindau antibody, Internal used in ELISA (ELISA). GTX89268
Anti-Von Hippel Lindau antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX89268
Anti-Von Hippel Lindau antibody, Internal used in Western Blot (WB). GTX89268

Cat. No. GTX89268

Reference 1

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, ELISA, Sandwich ELISA

Reactivity

 Human, Mouse, Rat
Package
100 μg ($399)
See all Von Hippel Lindau products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.01-0.03μg/ml
IHC-P 5μg/ml
ELISA Assay dependent
Sandwich ELISA Assay dependent
Not tested in other applications.

Calculated MW

24 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX89268-PEP).

Product Note

This antibody is expected to recognise both reported isoforms (NP_000542.1 and NP_937799.1).

Predict Reactivity

Dog(>80% identity)

PROPERTIES

Form

Liquid

Buffer

TBS, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-RSLVKPENYRRLD, from the internal region of the protein sequence according to NP_000542.1; NP_937799.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

von Hippel-Lindau tumor suppressor , HRCA1 , RCA1 , VHL1 , pVHL

Cellular Localization

Nucleus

Background

Von Hippel-Lindau syndrome (VHL) is a dominantly inherited familial cancer syndrome predisposing to a variety of malignant and benign tumors. A germline mutation of this gene is the basis of familial inheritance of VHL syndrome. The protein encoded by this gene is a component of the protein complex that includes elongin B, elongin C, and cullin-2, and possesses ubiquitin ligase E3 activity. This protein is involved in the ubiquitination and degradation of hypoxia-inducible-factor (HIF), which is a transcription factor that plays a central role in the regulation of gene expression by oxygen. RNA polymerase II subunit POLR2G/RPB7 is also reported to be a target of this protein. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Von Hippel Lindau antibody, Internal used in ELISA (ELISA). GTX89268

GTX89268 ELISA Image

ELISA analysis of Von Hippel Lindau protein using GTX89268 Von Hippel Lindau antibody, Internal.
Capture : GTX101087 (2.5μg/ml)
Detection : GTX89268 (1.5μg/ml)

Anti-Von Hippel Lindau antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX89268

GTX89268 IHC-P Image

IHC-P analysis of human kidney using GTX89268 Von Hippel Lindau antibody, Internal.
Antigen retrieval : citrate buffer pH 6
Dilution : 5μg/ml

Anti-Von Hippel Lindau antibody, Internal used in Western Blot (WB). GTX89268

GTX89268 WB Image

WB analysis of human ovary lysate using GTX89268 Von Hippel Lindau antibody, Internal.
Dilution : 0.01μg/ml
Loading : 35μg protein in RIPA buffer

REFERENCE

Application Reference

Yang KS et al. Sci Rep 2023; 13 (1) : 10969 Extracellular vesicle analysis of plasma allows differential diagnosis of atypical pancreatic serous cystadenoma.
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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399