APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:5000-1:20000 |
| Assay dependent |
Not tested in other applications.
Calculated MW
Product Note
This antibody is specific for human Wnt2 protein, and it does not cross react with human Wnt1, Wnt2b, Wnt3, Wnt3a, Wnt4, Wnt5a, Wnt6, Wnt7a, Wnt7b, Wnt8a, Wnt8b, Wnt9a, Wnt9b, Wnt10a, Wnt10b, and Wnt16 protein.
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant fragment of human Wnt2.
Purification
Affinity purified by Protein A.
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
Wnt family member 2 , INT1L1 , IRP
Cellular Localization
Secreted, extracellular space, extracellular matrix,Secreted
Background
This gene is a member of the WNT gene family. The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. Alternatively spliced transcript variants have been identified for this gene. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
![Anti-Wnt2 antibody [HL1999] used in Western Blot (WB). GTX637900](/templates/default/js/lightbox/images/loading.gif "Anti-Wnt2 antibody [HL1999] used in Western Blot (WB). GTX637900")
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GTX637900 WB Image
Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Wnt2 antibody [HL1999] (GTX637900) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX637900 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Wnt2 antibody [HL1999] (GTX637900) diluted at 1:15000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX637900 WB Image
Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Wnt2 antibody [HL1999] (GTX637900) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX637900 WB Image
Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Wnt2 antibody [HL1999] (GTX637900) diluted at 1:7500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX637900 ICC/IF Image
Wnt2 antibody [HL1999] detects Wnt2 protein by immunofluorescent analysis.Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Wnt2 stained by Wnt2 antibody [HL1999] (GTX637900) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
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GTX637900 WB Image
Whole zebrafish extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Wnt2 antibody [HL1999] (GTX637900) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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REFERENCE
There are currently no references for Wnt2 antibody [HL1999] (GTX637900). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Wnt2 antibody [HL1999] (GTX637900). Be the first to share your experience with this product.
![Anti-Wnt2 antibody [HL1999] used in Western Blot (WB). GTX637900](/upload/website/prouct_img/small/GTX637900/GTX637900_45103_20230721_WB_M_brain_23072519_799.webp "Anti-Wnt2 antibody [HL1999] used in Western Blot (WB). GTX637900")
![Anti-Wnt2 antibody [HL1999] used in Western Blot (WB). GTX637900](/upload/website/prouct_img/small/GTX637900/GTX637900_T-44872_20230512_WB_multiple_B_23051702_170.webp "Anti-Wnt2 antibody [HL1999] used in Western Blot (WB). GTX637900")
![Anti-Wnt2 antibody [HL1999] used in Western Blot (WB). GTX637900](/upload/website/prouct_img/small/GTX637900/GTX637900_T-44872_20230616_WB_R_brain_23062019_203.webp "Anti-Wnt2 antibody [HL1999] used in Western Blot (WB). GTX637900")
![Anti-Wnt2 antibody [HL1999] used in Western Blot (WB). GTX637900](/upload/website/prouct_img/small/GTX637900/GTX637900_45103_20231013_WB_C_23101921_102.webp "Anti-Wnt2 antibody [HL1999] used in Western Blot (WB). GTX637900")
![Anti-Wnt2 antibody [HL1999] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637900](/upload/website/prouct_img/small/GTX637900/GTX637900_45103_20231006_ICC_IF_B_Wnt2_23102401_568.webp "Anti-Wnt2 antibody [HL1999] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637900")
![Anti-Wnt2 antibody [HL1999] used in Western Blot (WB). GTX637900](/upload/website/prouct_img/small/GTX637900/GTX637900_45103_20230915_WB_Z_23091901_821.webp "Anti-Wnt2 antibody [HL1999] used in Western Blot (WB). GTX637900")
![Anti-Wnt2 antibody [HL1999] used in Western Blot (WB). GTX637900](/upload/website/prouct_img/normal/GTX637900/GTX637900_45103_20230721_WB_M_brain_23072519_799.webp "Anti-Wnt2 antibody [HL1999] used in Western Blot (WB). GTX637900")
![Anti-Wnt2 antibody [HL1999] used in Western Blot (WB). GTX637900](/upload/website/prouct_img/normal/GTX637900/GTX637900_T-44872_20230512_WB_multiple_B_23051702_170.webp "Anti-Wnt2 antibody [HL1999] used in Western Blot (WB). GTX637900")
![Anti-Wnt2 antibody [HL1999] used in Western Blot (WB). GTX637900](/upload/website/prouct_img/normal/GTX637900/GTX637900_T-44872_20230616_WB_R_brain_23062019_203.webp "Anti-Wnt2 antibody [HL1999] used in Western Blot (WB). GTX637900")
![Anti-Wnt2 antibody [HL1999] used in Western Blot (WB). GTX637900](/upload/website/prouct_img/normal/GTX637900/GTX637900_45103_20231013_WB_C_23101921_102.webp "Anti-Wnt2 antibody [HL1999] used in Western Blot (WB). GTX637900")
![Anti-Wnt2 antibody [HL1999] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637900](/upload/website/prouct_img/normal/GTX637900/GTX637900_45103_20231006_ICC_IF_B_Wnt2_23102401_568.webp "Anti-Wnt2 antibody [HL1999] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637900")
![Anti-Wnt2 antibody [HL1999] used in Western Blot (WB). GTX637900](/upload/website/prouct_img/normal/GTX637900/GTX637900_45103_20230915_WB_Z_23091901_821.webp "Anti-Wnt2 antibody [HL1999] used in Western Blot (WB). GTX637900")