APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:1000-1:10000 |
| Assay dependent |
| Assay dependent |
Not tested in other applications.
Calculated MW
Product Note
This antibody is specific for human Wnt8a protein, and it does not cross react with human Wnt1, Wnt2, Wnt2b, Wnt3, Wnt3a, Wnt4, Wnt5a, Wnt6, Wnt7a, Wnt7b, Wnt8b, Wnt9a, Wnt9b, Wnt10a, Wnt10b, and Wnt16 protein.
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant fragment of human Wnt8a
Purification
Affinity purified by Protein A.
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
Wnt family member 8A , WNT8D
Cellular Localization
Secreted, extracellular space, extracellular matrix,Secreted
Background
The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family, and may be implicated in development of early embryos as well as germ cell tumors. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2014]
Database
Research Area
DATA IMAGES
![Anti-Wnt8a antibody [HL1648] used in Western Blot (WB). GTX637229](/templates/default/js/lightbox/images/loading.gif "Anti-Wnt8a antibody [HL1648] used in Western Blot (WB). GTX637229")
|
GTX637229 WB Image
Whole zebrafish extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Wnt8a antibody [HL1648] (GTX637229) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
|
|
|
GTX637229 IHC-P Image
Wnt8a antibody [HL1648] detects Wnt8a protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded human colon cancer.Wnt8a stained by Wnt8a antibody [HL1648] (GTX637229) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
|
|
|
GTX637229 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with Wnt8a antibody [HL1648] (GTX637229) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
|
|
|
GTX637229 ICC/IF Image
Wnt8a antibody [HL1648] detects Wnt8a protein by immunofluorescent analysis.Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Wnt8a stained by Wnt8a antibody [HL1648] (GTX637229) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).Scale bar= 10μm.
|
REFERENCE
There are currently no references for Wnt8a antibody [HL1648] (GTX637229). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Wnt8a antibody [HL1648] (GTX637229). Be the first to share your experience with this product.
![Anti-Wnt8a antibody [HL1648] used in Western Blot (WB). GTX637229](/upload/website/prouct_img/small/GTX637229/GTX637229_45131_20230908_WB_Z_23091319_610.webp "Anti-Wnt8a antibody [HL1648] used in Western Blot (WB). GTX637229")
![Anti-Wnt8a antibody [HL1648] used in IHC (Paraffin sections) (IHC-P). GTX637229](/upload/website/prouct_img/small/GTX637229/GTX637229_T-44746_20230925_IHC-P_23100319_207.webp "Anti-Wnt8a antibody [HL1648] used in IHC (Paraffin sections) (IHC-P). GTX637229")
![Anti-Wnt8a antibody [HL1648] used in Western Blot (WB). GTX637229](/upload/website/prouct_img/small/GTX637229/GTX637229_T-44746_20230609_WB_multiple_B_23062019_750.webp "Anti-Wnt8a antibody [HL1648] used in Western Blot (WB). GTX637229")
![Anti-Wnt8a antibody [HL1648] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637229](/upload/website/prouct_img/small/GTX637229/GTX637229_45131_20231110_ICC_IF_B_23111422_551.webp "Anti-Wnt8a antibody [HL1648] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637229")
![Anti-Wnt8a antibody [HL1648] used in Western Blot (WB). GTX637229](/upload/website/prouct_img/normal/GTX637229/GTX637229_45131_20230908_WB_Z_23091319_610.webp "Anti-Wnt8a antibody [HL1648] used in Western Blot (WB). GTX637229")
![Anti-Wnt8a antibody [HL1648] used in IHC (Paraffin sections) (IHC-P). GTX637229](/upload/website/prouct_img/normal/GTX637229/GTX637229_T-44746_20230925_IHC-P_23100319_207.webp "Anti-Wnt8a antibody [HL1648] used in IHC (Paraffin sections) (IHC-P). GTX637229")
![Anti-Wnt8a antibody [HL1648] used in Western Blot (WB). GTX637229](/upload/website/prouct_img/normal/GTX637229/GTX637229_T-44746_20230609_WB_multiple_B_23062019_750.webp "Anti-Wnt8a antibody [HL1648] used in Western Blot (WB). GTX637229")
![Anti-Wnt8a antibody [HL1648] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637229](/upload/website/prouct_img/normal/GTX637229/GTX637229_45131_20231110_ICC_IF_B_23111422_551.webp "Anti-Wnt8a antibody [HL1648] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637229")