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ZO-1 antibody [HL1133]

Recombinant antibody KO/KD
Anti-ZO-1 antibody [HL1133] used in Western Blot (WB). GTX636399
Anti-ZO-1 antibody [HL1133] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636399
Anti-ZO-1 antibody [HL1133] used in Western Blot (WB). GTX636399

Cat. No. GTX636399

Reference 1

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL1133

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all ZO-1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
Not tested in other applications.

Calculated MW

195 kDa. ( Note )

Product Note

We do not recommend use of this product for Mouse,Rat samples.

Predict Reactivity

Mouse, Rat, Dog(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human ZO-1. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2909975

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

tight junction protein 1 , ZO-1

Cellular Localization

Cell membrane,Cell junction

Background

This gene encodes a member of the membrane-associated guanylate kinase (MAGUK) family of proteins, and acts as a tight junction adaptor protein that also regulates adherens junctions. Tight junctions regulate the movement of ions and macromolecules between endothelial and epithelial cells. The multidomain structure of this scaffold protein, including a postsynaptic density 95/disc-large/zona occludens (PDZ) domain, a Src homology (SH3) domain, a guanylate kinase (GuK) domain and unique (U) motifs all help to co-ordinate binding of transmembrane proteins, cytosolic proteins, and F-actin, which are required for tight junction function. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Aug 2017]

Database

Research Area

DATA IMAGES

Anti-ZO-1 antibody [HL1133] used in Western Blot (WB). GTX636399

GTX636399 WB Image

KO/KD

Wild-type (WT) and TJP1 knockout (KO) HeLa cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ZO-1 antibody [HL1133] (GTX636399) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ZO-1 antibody [HL1133] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636399

GTX636399 ICC/IF Image

ZO-1 antibody [HL1133] detects ZO-1 protein at cell membrane by immunofluorescent analysis.
Sample: MCF-7 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ZO-1 stained by ZO-1 antibody [HL1133] (GTX636399) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-ZO-1 antibody [HL1133] used in Western Blot (WB). GTX636399

GTX636399 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ZO-1 antibody [HL1133] (GTX636399) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Jin B et al. Front Med (Lausanne) 2023; 10 : 1213191 Kunxian capsule alleviates podocyte injury and proteinuria by inactivating β-catenin in db/db mice.
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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations