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mTOR antibody [HL2216]

Recombinant antibody KO/KD Orthogonal
Anti-mTOR antibody [HL2216] used in Western Blot (WB). GTX638220
Anti-mTOR antibody [HL2216] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638220
Anti-mTOR antibody [HL2216] used in Western Blot (WB). GTX638220
Anti-mTOR antibody [HL2216] used in Western Blot (WB). GTX638220
Anti-mTOR antibody [HL2216] used in Western Blot (WB). GTX638220
Anti-mTOR antibody [HL2216] used in Western Blot (WB). GTX638220
Anti-mTOR antibody [HL2216] used in IHC (Paraffin sections) (IHC-P). GTX638220

Cat. No. GTX638220

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL2216

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all mTOR products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

289 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragment of human mTOR.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

mechanistic target of rapamycin kinase , FRAP , FRAP1 , FRAP2 , RAFT1 , RAPT1 , SKS

Cellular Localization

Endoplasmic reticulum membrane , Golgi apparatus membrane , Mitochondrion outer membrane , Lysosome , Cytoplasm

Background

The protein encoded by this gene belongs to a family of phosphatidylinositol kinase-related kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. The ANGPTL7 gene is located in an intron of this gene. [provided by RefSeq, Sep 2008]

Database

Research Area

DATA IMAGES

Anti-mTOR antibody [HL2216] used in Western Blot (WB). GTX638220

GTX638220 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with mTOR antibody [HL2216] (GTX638220) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-mTOR antibody [HL2216] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638220

GTX638220 ICC/IF Image

mTOR antibody [HL2216] detects mTOR protein at Golgi apparatus and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: mTOR stained by mTOR antibody [HL2216] (GTX638220) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-mTOR antibody [HL2216] used in Western Blot (WB). GTX638220

GTX638220 WB Image

Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with mTOR antibody [HL2216] (GTX638220) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-mTOR antibody [HL2216] used in Western Blot (WB). GTX638220

GTX638220 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with mTOR antibody [HL2216] (GTX638220) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-mTOR antibody [HL2216] used in Western Blot (WB). GTX638220

GTX638220 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with mTOR antibody [HL2216] (GTX638220) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-mTOR antibody [HL2216] used in Western Blot (WB). GTX638220

GTX638220 WB Image

Various tissue extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with mTOR antibody [HL2216] (GTX638220) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.

Anti-mTOR antibody [HL2216] used in IHC (Paraffin sections) (IHC-P). GTX638220

GTX638220 IHC-P Image

mTOR antibody [HL2216] detects mTOR protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse duodenum.
mTOR stained by mTOR antibody [HL2216] (GTX638220) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations